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mouse monoclonal antibody 9d5 against scap  (ATCC)


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    Structured Review

    ATCC mouse monoclonal antibody 9d5 against scap
    LY295427 binds to INSIG. A: The structure of the LY295427 probe and a schematic illustration of the detection procedure for LY295427 -binding proteins. B: CHO cells were incubated in cholesterol depletion medium supplemented with or without 20 μM LY295427 , 20 μM LY295427 probe, and 1 μg/ml 25-HC for 24 h. The cells were harvested and subjected to immunoblotting. C–G: CHO cells were transfected with pCMV-INSIG-1-Myc, pCMV-INSIG-2-Myc, or pCMV-INSIG-2 (F115A)-Myc plasmid for 24 h and incubated in cholesterol depletion medium supplemented with or without LY295427 probe, LY295427 , and 25-HC for 24 h. The cells were harvested and treated as described in A. Input and pellet were immunoblotted with anti-Myc antibody. H: HEK293T cells were transfected with pLVX-SCAP-EGFP or pLVX-SCAP(D443N)-EGFP plasmid for 24 h and incubated in cholesterol depletion medium supplemented with or without LY295427 probe and cholesterol/CDX for 24 h. The cells were harvested and treated as described in A. Input and pellet were immunoblotted with anti-Myc and anti-SCAP <t>(9D5)</t> antibodies. I: The model diagram illustrating the action of 25-HC and LY295427 .
    Mouse Monoclonal Antibody 9d5 Against Scap, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+antibody+9d5+against+scap/pmc13090600-18-45-52?v=ATCC
    Average 94 stars, based on 19 article reviews
    mouse monoclonal antibody 9d5 against scap - by Bioz Stars, 2026-08
    94/100 stars

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    1) Product Images from "The compound LY295427 antagonizes 25-hydroxycholesterol through binding to INSIG"

    Article Title: The compound LY295427 antagonizes 25-hydroxycholesterol through binding to INSIG

    Journal: Journal of Lipid Research

    doi: 10.1016/j.jlr.2026.101015

    LY295427 binds to INSIG. A: The structure of the LY295427 probe and a schematic illustration of the detection procedure for LY295427 -binding proteins. B: CHO cells were incubated in cholesterol depletion medium supplemented with or without 20 μM LY295427 , 20 μM LY295427 probe, and 1 μg/ml 25-HC for 24 h. The cells were harvested and subjected to immunoblotting. C–G: CHO cells were transfected with pCMV-INSIG-1-Myc, pCMV-INSIG-2-Myc, or pCMV-INSIG-2 (F115A)-Myc plasmid for 24 h and incubated in cholesterol depletion medium supplemented with or without LY295427 probe, LY295427 , and 25-HC for 24 h. The cells were harvested and treated as described in A. Input and pellet were immunoblotted with anti-Myc antibody. H: HEK293T cells were transfected with pLVX-SCAP-EGFP or pLVX-SCAP(D443N)-EGFP plasmid for 24 h and incubated in cholesterol depletion medium supplemented with or without LY295427 probe and cholesterol/CDX for 24 h. The cells were harvested and treated as described in A. Input and pellet were immunoblotted with anti-Myc and anti-SCAP (9D5) antibodies. I: The model diagram illustrating the action of 25-HC and LY295427 .
    Figure Legend Snippet: LY295427 binds to INSIG. A: The structure of the LY295427 probe and a schematic illustration of the detection procedure for LY295427 -binding proteins. B: CHO cells were incubated in cholesterol depletion medium supplemented with or without 20 μM LY295427 , 20 μM LY295427 probe, and 1 μg/ml 25-HC for 24 h. The cells were harvested and subjected to immunoblotting. C–G: CHO cells were transfected with pCMV-INSIG-1-Myc, pCMV-INSIG-2-Myc, or pCMV-INSIG-2 (F115A)-Myc plasmid for 24 h and incubated in cholesterol depletion medium supplemented with or without LY295427 probe, LY295427 , and 25-HC for 24 h. The cells were harvested and treated as described in A. Input and pellet were immunoblotted with anti-Myc antibody. H: HEK293T cells were transfected with pLVX-SCAP-EGFP or pLVX-SCAP(D443N)-EGFP plasmid for 24 h and incubated in cholesterol depletion medium supplemented with or without LY295427 probe and cholesterol/CDX for 24 h. The cells were harvested and treated as described in A. Input and pellet were immunoblotted with anti-Myc and anti-SCAP (9D5) antibodies. I: The model diagram illustrating the action of 25-HC and LY295427 .

    Techniques Used: Binding Assay, Incubation, Western Blot, Transfection, Plasmid Preparation



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    mouse monoclonal antibody 9d5 against scap  (ATCC)
    94
    ATCC mouse monoclonal antibody 9d5 against scap
    LY295427 binds to INSIG. A: The structure of the LY295427 probe and a schematic illustration of the detection procedure for LY295427 -binding proteins. B: CHO cells were incubated in cholesterol depletion medium supplemented with or without 20 μM LY295427 , 20 μM LY295427 probe, and 1 μg/ml 25-HC for 24 h. The cells were harvested and subjected to immunoblotting. C–G: CHO cells were transfected with pCMV-INSIG-1-Myc, pCMV-INSIG-2-Myc, or pCMV-INSIG-2 (F115A)-Myc plasmid for 24 h and incubated in cholesterol depletion medium supplemented with or without LY295427 probe, LY295427 , and 25-HC for 24 h. The cells were harvested and treated as described in A. Input and pellet were immunoblotted with anti-Myc antibody. H: HEK293T cells were transfected with pLVX-SCAP-EGFP or pLVX-SCAP(D443N)-EGFP plasmid for 24 h and incubated in cholesterol depletion medium supplemented with or without LY295427 probe and cholesterol/CDX for 24 h. The cells were harvested and treated as described in A. Input and pellet were immunoblotted with anti-Myc and anti-SCAP <t>(9D5)</t> antibodies. I: The model diagram illustrating the action of 25-HC and LY295427 .
    Mouse Monoclonal Antibody 9d5 Against Scap, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+antibody+9d5+against+scap/pmc13090600-18-45-52?v=ATCC
    Average 94 stars, based on 1 article reviews
    mouse monoclonal antibody 9d5 against scap - by Bioz Stars, 2026-08
    94/100 stars
    		  Buy from Supplier

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    LY295427 binds to INSIG. A: The structure of the LY295427 probe and a schematic illustration of the detection procedure for LY295427 -binding proteins. B: CHO cells were incubated in cholesterol depletion medium supplemented with or without 20 μM LY295427 , 20 μM LY295427 probe, and 1 μg/ml 25-HC for 24 h. The cells were harvested and subjected to immunoblotting. C–G: CHO cells were transfected with pCMV-INSIG-1-Myc, pCMV-INSIG-2-Myc, or pCMV-INSIG-2 (F115A)-Myc plasmid for 24 h and incubated in cholesterol depletion medium supplemented with or without LY295427 probe, LY295427 , and 25-HC for 24 h. The cells were harvested and treated as described in A. Input and pellet were immunoblotted with anti-Myc antibody. H: HEK293T cells were transfected with pLVX-SCAP-EGFP or pLVX-SCAP(D443N)-EGFP plasmid for 24 h and incubated in cholesterol depletion medium supplemented with or without LY295427 probe and cholesterol/CDX for 24 h. The cells were harvested and treated as described in A. Input and pellet were immunoblotted with anti-Myc and anti-SCAP (9D5) antibodies. I: The model diagram illustrating the action of 25-HC and LY295427 .

    Journal: Journal of Lipid Research

    Article Title: The compound LY295427 antagonizes 25-hydroxycholesterol through binding to INSIG

    doi: 10.1016/j.jlr.2026.101015

    Figure Lengend Snippet: LY295427 binds to INSIG. A: The structure of the LY295427 probe and a schematic illustration of the detection procedure for LY295427 -binding proteins. B: CHO cells were incubated in cholesterol depletion medium supplemented with or without 20 μM LY295427 , 20 μM LY295427 probe, and 1 μg/ml 25-HC for 24 h. The cells were harvested and subjected to immunoblotting. C–G: CHO cells were transfected with pCMV-INSIG-1-Myc, pCMV-INSIG-2-Myc, or pCMV-INSIG-2 (F115A)-Myc plasmid for 24 h and incubated in cholesterol depletion medium supplemented with or without LY295427 probe, LY295427 , and 25-HC for 24 h. The cells were harvested and treated as described in A. Input and pellet were immunoblotted with anti-Myc antibody. H: HEK293T cells were transfected with pLVX-SCAP-EGFP or pLVX-SCAP(D443N)-EGFP plasmid for 24 h and incubated in cholesterol depletion medium supplemented with or without LY295427 probe and cholesterol/CDX for 24 h. The cells were harvested and treated as described in A. Input and pellet were immunoblotted with anti-Myc and anti-SCAP (9D5) antibodies. I: The model diagram illustrating the action of 25-HC and LY295427 .

    Article Snippet: The primary antibodies used were as follows: mouse monoclonal antibody against β-actin (#A5441; Sigma), mouse monoclonal antibody against FLAG-tag (#F3165; Sigma), mouse monoclonal antibody against T7-tag (#69522; Merck), mouse monoclonal antibody against HA-tag (#H3663; Sigma), mouse monoclonal antibody P4D1 against ubiquitin (#SC-8017; Santa Cruz Biotechnology), mouse monoclonal antibody 9D5 against SCAP (#CRL-2347; ATCC), mouse monoclonal antibody 7D4 against SREBP2 (#CRL-2198; ATCC), anti-farnesyl-diphosphate farnesyltransferase 1 (FDFT1) antibody (#13128; Proteintech), anti-lanosterol synthase (LSS) antibody (#18693; Proteintech), mouse monoclonal antibody 9E10 against MYC-tag, mouse monoclonal antibody A9 against HMGCR, mouse monoclonal antibody 1D2 against SREBP2, rabbit polyclonal antibody against enhanced GFP (EGFP)-tag, rabbit polyclonal antibody against LDLR, and rabbit polyclonal antibody against HMGCR, which were prepared in our laboratory.

    Techniques: Binding Assay, Incubation, Western Blot, Transfection, Plasmid Preparation